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KMID : 0043320090320010091
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Archives of Pharmacal Research
2009 Volume.32 No. 1 p.91 ~ p.98
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Inhibitory Effects of an Aqueous Extract of Cornus kousa Burg. Leaves on TNF-α-Induced Chemokine Expression and Monocyte Adhesion to Human Colonic Epithelial Cells
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Kim Jung-Ae
Kim Ah-Ra
Park Su-Young
Lee Jong-Suk
Thapa Dinesh
Babu Dinesh
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Abstract
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An aqueous extract of Cornus kousa Burg. leaves (ACK) that contained high amount of polyphenols showed significant antioxidant activity against diphenylpicrylhydrazyl (DPPH) radicals and TNF-α-generated reactive oxygen species. ACK at concentrations of 10 and 50 μg/mL significantly inhibited TNF- α-induced adhesion of U937 pre-monocytic cells to HT-29 colon epithelial cells in a concentrationdependent manner. The reduced adhesion by ACK correlated with the suppressed expressions of monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8, the major inflammatory bowel disease (IBD)-associated chemokines. Moreover, ACK significantly suppressed TNF-α-induced translocation of redox-sensitive nuclear factor (NF)-κB as well as degradation of cytosolic I-κBα. The effective concentrations of ACK were much lower than that of 5-aminosalicylic acid (3.06 mg/mL), which is an active metabolite of sulfasalazine, a well-known drug used in the treatment of IBD. The results indicate that ACK may provide a potential benefit for the prevention and treatment of inflammatory diseases such as IBD.
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KEYWORD
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TNF-α, Intestinal inflammation, Chemokine, Cornus kousa Burg., NF-κB, 5-Aminosalicylic acid
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